Cloning and sequence analysis of a class A beta-lactamase from Mycobacterium tuberculosis H37Ra.
نویسندگان
چکیده
A cosmid library from Mycobacterium tuberculosis H37Ra was introduced into Mycobacterium smegmatis, and eight recombinant clones with increased resistance to cefoxitin were identified. Isoelectric focusing detected an M. tuberculosis-derived beta-lactamase in one of these recombinant clones. A sequence analysis identified it as a class A beta-lactamase whose expression correlated with the increased resistance phenotype.
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 41 5 شماره
صفحات -
تاریخ انتشار 1997